Invitrogen

NGAL Monoclonal Antibody (29)

NGAL Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

NGAL Antibody (ABS 043-29-02) in ICC/IF

Immunofluorescence analysis of NGAL was performed using 70% confluent log phase RAW 264.7 treated with Thapsigargin (250 nm, 48hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NGAL Monoclonal Antibody (29) (Product # ABS 043-29-02) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody... View More

NGAL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

NGAL Antibody — Detection of altered subcellular localization of the target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis using NGAL Monoclonal Antibody (29) (Product # ABS 043-29-02), shows an increase in expression of NGAL in RAW 264.7 upon Thapsigargin treatment. {TM}

Product Details

ABS 043-29-02

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

Immunocytochemistry (ICC/IF)

5 µg/mL

ELISA (ELISA)

1:4,000

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

Immunogen

Recombinant mouse NGAL adsorbed onto aluminum hydroxide gel.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.92 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2136588

Product Specific Information

ABS 043-29-02 binds free NGAL in mouse serum.

This antibody will not cross react with human, pig, dog, or rat.

ABS 043-29-02 may be used in Western blot and ELISA applications.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Target Information

NGAL (Neutrophil gelatinase-associated lipocalin, lipocalin 2, siderocalin and neutrophil lipocalin) is a 25 kDa glycosylated single chain monomer and member of the lipocalin family of proteins that bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, can form dimers and small amounts of higher oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by ischemic or nephrotoxic kidney injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Zebrafish GSDMEb Cleavage-Gated Pyroptosis Drives Septic Acute Kidney Injury In Vivo.

Journal of immunology (Baltimore, Md. : 1950)

Wang Z,Gu Z,Hou Q,Chen W,Mu D,Zhang Y,Liu Q,Liu Z,Yang D

ABS 043-29-02 was used in Western Blot to use a zebrafish crispant in vivo analysis model to investigate LPS-induced septic shock.

Wed Apr 01 00:00:00 UTC 2020

Pulsed focused ultrasound enhances the therapeutic effect of mesenchymal stromal cell-derived extracellular vesicles in acute kidney injury.

Stem cell research & therapy

Ullah M,Liu DD,Rai S,Razavi M,Concepcion W,Thakor AS

ABS 043-29-02 was used in Western Blot to show that pFUS may be a strategy for enhancing the therapeutic efficacy of MSC-derived EV treatment for the treatment of AKI.

Mon Sep 14 00:00:00 UTC 2020

Pulsed focused ultrasound enhances the therapeutic effect of mesenchymal stromal cell-derived extracellular vesicles in acute kidney injury.

Stem cell research & therapy

Ullah M,Liu DD,Rai S,Razavi M,Concepcion W,Thakor AS

ABS 043-29-02 was used in Western Blot to show that pFUS may be a strategy for enhancing the therapeutic efficacy of MSC-derived EV treatment for the treatment of AKI.

Mon Sep 14 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: 24p3; HGNC:6526; Lipocalin-2; Lipocalin2; Neutrophil gelatinase-associated lipocalin; NGAL; Oncogene 24p3; OTTHUMP00000022240; p25; RP23-161B9.11; secreted inducible protein 24; Siderocalin LCN2; SV-40-induced 24P3 protein; uterocalin

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Gene Aliases: 24p3; AW212229; Lcn2; Sip24

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UniProt ID: (Mouse) P11672

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Entrez Gene ID: (Mouse) 16819

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Function(s)

protease binding transporter activity iron ion binding protein binding small molecule binding protein homodimerization activity

Process(es)

immune system process transport ion transport apoptotic process response to virus response to herbicide positive regulation of gene expression siderophore transport positive regulation of cell projection organization response to drug innate immune response iron ion homeostasis protein homotrimerization cellular response to hydrogen peroxide extrinsic apoptotic signaling pathway in absence of ligand

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NGAL Monoclonal Antibody (29)

NGAL Antibody (ABS 043-29-02) in ICC/IF

Product Details

ABS 043-29-02

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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